Mouse Anti-EREG Recombinant Antibody (clone 9E5)
CAT#: PABL-084
Recombinant Mouse Antibody (9E5) is capable of binding to Epiregulin, expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Tested Data
Gene Expression
Figure 3 Immunofluorescent staining of antibodies in human colorectal carcinoma cells.
DLD-1 and HCT116 cells, representing two human colorectal carcinoma cell lines, were fixed with paraformaldehyde and treated with or without 0.2% Triton X-100 solution (localization to endomembranes was observed; white arrow).
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
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SDS-PAGE
Figure 1 Mouse Anti-EREG Recombinant Antibody (PABL-084) in SDS-PAGE
SDS-PAGE analysis of PABL-084 in non-reduced (Lane 1, 2 μg) and reduced (Lane 2, 2 μg) conditions.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54373/if_selected.jpg
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Specifications
- Immunogen
- Human epiregulin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EREG
- Species Reactivity
- Human
- Clone
- 9E5
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence and Flow Cytometry. For details, refer to published data.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-084, RRID: AB_3111626)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 9E5"
- CAT
- Product Name
See other products for "EREG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2396z | Mouse Anti-EREG Recombinant Antibody (clone 43A4) | WB, IHC | Mouse IgG1 |
| MOB-0693CT | Recombinant Mouse anti-Human EREG Monoclonal antibody (NN0376- 23Z5) | WB | |
| VS-0723-WK135 | Human Anti-EREG Recombinant Antibody (VS-0723-WK135) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-084 | Mouse Anti-EREG Recombinant Antibody (clone 9E5); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-084 | Mouse Anti-EREG Recombinant Antibody (clone 9E5); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-004WM | Anti-Human EREG Recombinant Antibody (EP27) | ELISA, Neut | Humanized Antibody |
| TAB-004WM-S(P) | Anti-Human EREG Recombinant Antibody scFv Fragment (EP27) | ELISA, Neut | Humanized Antibody |
| TAB-004WM-F(E) | Anti-Human EREG Recombinant Antibody Fab Fragment (EP27) | ELISA, Neut | Humanized Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-745CQ | Rat Anti-Ereg Recombinant Antibody (clone 6H12) | Neut, ELISA | Rat IgG2 |
| NEUT-746CQ | Rat Anti-Ereg Recombinant Antibody (clone CBL583) | ELISA, Neut | Rat IgG2a |
| NEUT-747CQ | Rat Anti-Ereg Recombinant Antibody (clone CBL870) | ELISA, Neut | Rat IgG |
| NEUT-748CQ | Rat Anti-Ereg Recombinant Antibody (clone RM0233-6H12) | Neut | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC370 | Recombinant Anti-EREG Vesicular Antibody, EV Displayed (VS-0425-YC370) | ELISA, FC, Neut, Cell-uptake |
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