Mouse Anti-EREG Recombinant Antibody (clone 9E5)
CAT#: PABL-084
Recombinant Mouse Antibody (9E5) is capable of binding to Epiregulin, expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Tested Data
Gene Expression
Figure 3 Immunofluorescent staining of antibodies in human colorectal carcinoma cells.
DLD-1 and HCT116 cells, representing two human colorectal carcinoma cell lines, were fixed with paraformaldehyde and treated with or without 0.2% Triton X-100 solution (localization to endomembranes was observed; white arrow).
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
Figure 4 Flow cytometry analysis.
Antibodies were tested for their ability bind to the surface of each cell line (DLD-1, HCT116, and AGS). Green line: 0.1 lg/mL, blue line: 0.5 lg/mL, purple line: 1 lg/mL, red line: negative control (0 lg/mL).
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
Figure 5 Single-cycle kinetic analysis of 9E5, HM0, and HM1 antibody binding to an amine-coupled CM5 chip on Biacore.
hEpiregulin-Fc was injected at concentrations of 1.5 lM, 300, 60, 12, and 2.4 nM, respectively, and analyzed in duplicate binding cycles over three differing capacity reaction surfaces.
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
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SDS-PAGE
Figure 1 Mouse Anti-EREG Recombinant Antibody (PABL-084) in SDS-PAGE
SDS-PAGE analysis of PABL-084 in non-reduced (Lane 1, 2 μg) and reduced (Lane 2, 2 μg) conditions.
SEC-HPLC
Figure 2 Mouse Anti-EREG Recombinant Antibody (PABL-084) in HPLC
The purity of PABL-084 was greater than 98% as determined by SEC-HPLC.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54373/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000124882-EREG
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Specifications
- Immunogen
- Human epiregulin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EREG
- Species Reactivity
- Human
- Clone
- 9E5
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence and Flow Cytometry. For details, refer to published data.
Target
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(Creative Biolabs Cat# PABL-084, RRID: AB_3111626)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone 9E5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2396z | Mouse Anti-EREG Recombinant Antibody (clone 43A4) | WB, IHC | Mouse IgG1 |
| MOB-0693CT | Recombinant Mouse anti-Human EREG Monoclonal antibody (NN0376- 23Z5) | WB | |
| VS-0723-WK135 | Human Anti-EREG Recombinant Antibody (VS-0723-WK135) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-084 | Mouse Anti-EREG Recombinant Antibody (clone 9E5); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-084 | Mouse Anti-EREG Recombinant Antibody (clone 9E5); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-004WM | Anti-Human EREG Recombinant Antibody (EP27) | ELISA, Neut | Humanized Antibody |
| TAB-004WM-S(P) | Anti-Human EREG Recombinant Antibody scFv Fragment (EP27) | ELISA, Neut | Humanized Antibody |
| TAB-004WM-F(E) | Anti-Human EREG Recombinant Antibody Fab Fragment (EP27) | ELISA, Neut | Humanized Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-745CQ | Rat Anti-Ereg Recombinant Antibody (clone 6H12) | Neut, ELISA | Rat IgG2 |
| NEUT-746CQ | Rat Anti-Ereg Recombinant Antibody (clone CBL583) | ELISA, Neut | Rat IgG2a |
| NEUT-747CQ | Rat Anti-Ereg Recombinant Antibody (clone CBL870) | ELISA, Neut | Rat IgG |
| NEUT-748CQ | Rat Anti-Ereg Recombinant Antibody (clone RM0233-6H12) | Neut | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC370 | Recombinant Anti-EREG Vesicular Antibody, EV Displayed (VS-0425-YC370) | ELISA, FC, Neut, Cell-uptake |
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